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. 2021 Jul 1;25(15):7367–7380. doi: 10.1111/jcmm.16765

FIGURE 5.

FIGURE 5

HMGA2 was a direct target of miR‐515‐5p. A, The binding of miR‐515‐5p with HMGA2 gene 3’‐UTR predicted by TargetScan. B, The direct binding between miR‐515‐5p and HMGA2 gene promoter was performed by dual‐luciferase reporter assay. C, HMGA2 mRNA levels in HL60 and THP‐1 cells transfected with miR‐515‐5p mimics. D, HMGA2 protein levels in HL60 and THP‐1 cells transfected with miR‐515‐5p mimics. E, The anti‐miR‐515‐5p decreased the miR‐515‐5p levels in HL60 and THP‐1 cells. F, QRT‐PCR assay confirmed that knockdown of circTASP1 reduced HMGA2 expression, which was reversed by anti‐miR‐515‐5p. G, Western blot confirmed that knockdown of circTASP1 reduced HMGA2 expression, which was reversed by anti‐miR‐515‐5p. H, QRT‐PCR assay proved that HMGA2 expression in patients with AML was higher than that in normal individuals. I, Negative correlation between miR‐515‐5p and HMGA2 expression in patients with AML. Positive correlation between circTASP1 and HMGA2 expression in patients with AML. **P < .01, ##P < .01. Each experiment was repeated 3 times