TABLE 1.

Forward and reverse primer sequences (5′‐3′) used in quantitative RT‐PCR

Target gene	Forward	Reverse
NRF2	5′‐ GGC TCA GCA CCT TGT ATC TT ‐3′	5′‐ CAC ATT GCC ATC TCT GGT TTG ‐3′
NQO‐1	5′‐ GAG AAG AGC CCT GAT TGT ACT G ‐3′	5′‐ ACC TCC CAT CCT CTC TTC TT ‐3′
HO‐1	5′‐ CTC CCT GTG TTT CCT TTC TCT C ‐3′	5′‐ GCT GCT GGT TTC AAA GTT CAG ‐3′
NF‐kB	5′‐ AGA CAT CCT TCC GCA AAC TC ‐3′	5′‐ TAG GTC CTT CCT GCC CAT AA ‐3′
Claudin‐5	5′‐ GGT GAA GTA GGC ACC AAA CT ‐3′	5′‐ TTT CTC CAG CTG CCC TTT C ‐3′
Occludin	5′‐ CAG CAG CAA TGG TAA CCT AGA G ‐3′	5′‐ CAC CTG TCG TGT AGT CTG TTT C ‐3′
VCAM‐1	5′‐ GAG GGA GAC ACC GTC ATT ATC ‐3′	5′‐ CGA GCC ATC CAC AGA CTT TA ‐3′
PECAM‐1	5′‐ CAA CAG AGC CAG CAG TAT GA ‐3′	5′‐ TGA CAA CCA CCG CAA TGA ‐3′
ZO‐1	5′‐ CAT TAC GAC CCT GAA GAG GAT G ‐3′	5′‐ AGC AGG AAG ATG TGC AGA AG ‐3′
Β‐Actin	5′‐ GAG GTA TCC TGA CCC TGA AGT A ‐3′	5′‐ CAC ACG CAG CTC ATT GTA GA ‐3′