FIGURE 2.

PYO up‐regulates HMGN2ac in macrophages. RAW 264.7 cells were treated with 50 μM PYO or DMSO for 6 h, and TSA (50 nM) was used as a positive control for HMGN2ac. ⁴⁹ (A) IP showing the acetylation of lysine (ACE‐lysine) with (B) densitometric analysis showing the relative expression normalized by that of the DMSO group. (C) IP showing the HMGN2 with (D) densitometric analysis showing the relative expression normalized by the DMSO group. (E) The LC/MS mass spectrometer displaying the acetylation modification sites of HMGN2, and the amino acid sequences of HMGN2 of different species were compared and the acetylation sites were marked. NLS: nuclear localization signal domain, NBD: nucleosome binding domain and RD: regulatory domain. PM and THP‐1 cells were treated both with 50 μM PYO and with DMSO for 6 h, (F) IP HMGN2 showing the ACE‐lysine with (G) the densitometric analysis showing relative expression normalized by DMSO group. Data are expressed as mean ± SD, *P < 0.05 compared with the DMSO group, n = 3