FIGURE 2.

Overexpression of miR27a in nucleus pulposus cells inhibits apoptosis and the production of inflammatory cytokines. (A) The transfection efficiency of miR27a as detected by qPCR. (B, C, D, E and F) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, COX2 expression was detected by immunofluorescence (B, C), qPCR (D) and Western blotting (E, F). (E, G, H, I) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, Bcl2, Bax and caspase 3 expression was detected by Western blotting. (J, K) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, apoptosis was detected by flow cytometry. Statistical analysis was based on at least three different biological samples and three technical replicates; P < .05 was considered to be statistically significant. Representative images are shown