FIGURE 3.

Overexpression of miR27a inhibits the production of matrix metalloproteinases and increases the deposition of extracellular matrix. (A, B, C, D) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 24 h, MMP13 expression was detected by immunofluorescence (A), qPCR (B) and Western blotting (C, D). (E) NP cells were transfected with miR27a mimics or NC for 24 h and then treated with 10 ng/mL TNF‐α. After 3 d, extracellular matrix deposition was detected by Alcian blue staining. Statistical analysis was based on at least three different biological samples and three technical replicates; P < .05 was considered to be statistically significant. Representative images are shown