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. 2021 Jul 22;24(3):675. doi: 10.3892/mmr.2021.12314

Figure 2.

Figure 2.

miR-140-5p regulates cell injury in AML12 cells exposed to H/R. (A) Transfection efficiency of miR-140-5p mimics and inhibitor was assessed by RT-qPCR. (B) Effects of miR-140-5p mimics and inhibitors on cell viability after H/R were analyzed by Cell Counting Kit-8 assay. (C) Effects of miR-140-5p mimics and inhibitors on lactate dehydrogenase release after H/R. (D-F) Inflammatory cytokine levels of IL-1β, IL-6, and tumor necrosis factor-α were detected by RT-qPCR. (G and H) Cell apoptosis was assessed by flow cytometry. (I) Western blot analysis and (J) quantification of the protein levels of Bax, Bcl-2 and cleaved caspase-3. *P<0.05 and **P<0.01 vs. the control group; #P<0.05 and ##P<0.01 vs. the H/R group. miR, microRNA; H/R, hypoxia reoxygenation; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LDH, lactate dehydrogenase; IL, interleukin; TNF, tumor necrosis factor.