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Primer sequences for reverse transcription-quantitative PCR.
| Gene | Primer sequence (5′-3′) |
|---|---|
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT | |
| miR-140-5p | F: CGCGCAGTGGTTTTACCCTA |
| R: AGTGCAGGGTCCGAGGTATT | |
| IL-6 | F: AGAGACTTCCATCCAGTTGCC |
| R: TCCTCTGTGAAGTCTCCTCTCC | |
| IL-1β | F: GCTTCAGGCAGGCAGTATCA |
| R: AGTCACAGAGGATGGGCTCT | |
| TNF-α | F: AGCCGATGGGTTGTACCTTG |
| R: ATAGCAAATCGGCTGACGGT |
F, forward; R, reverse; miR, microRNA; IL, interleukin, TNF, tumor necrosis factor.
