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. 2021 Jul 21;24(3):672. doi: 10.3892/mmr.2021.12311

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — DI inhibits inflammasome-mediated pyroptosis through autophagy. (A) Autophagy markers were detected by western blotting. Protein expression levels of (B) LC3B, (C) Beclin-1, (D) ATG-5 and (E) P62 were semi-quantified. (F) Association between DI-induced autophagy and inflammation was investigated by western blotting. Protein expression levels of (G) GSDMD, (H) cleaved GSDMD, (I) NLRP3 and (J) pro-IL-1β were semi-quantified. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. sham group; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. LPS+ATP group or as indicated. 3-MA, 3-methyladenine; ATG-5, autophagy-related 5; DI, dimethyl itaconate; GSDMD, gasdermin D; LPS, lipopolysaccharide; NLRP3, NLR family pyrin domain-containing 3.