a, Comparison of TRPV125C,CAP (green) and TRPV148C,CAP,INT (orange) viewed from the membrane. Arrows indicate movements of the ARD, CD, S1-S4 domain, and TRP helix. b, The same as (a), viewed from the intracellular side. ARD/CD movement occurs at an individual protomer level. c, TRPV148C,CAP,INT and TRPV148C,CAP,OPEN adopt similar overall conformations. d, Close-up view of TRPV125C,CAP and TRPV148C,CAP,INT cytoplasmic domains. e, Close-up view of TRPV125C,CAP and TRPV148C,CAP,INT S1-S4 domain. f, Close-up view of the S6b and TRP helix of TRPV125C,CAP, TRPV148C,CAP,INT, and TRPV148C,CAP,OPEN . The arrows indicate TRP helix and S6b movements.