FIGURE 2.

Knockdown of HHLA2 inhibited NSCLC cell proliferation and cell cycle progression. (A) CCK8 assay revealed that HHLA2 silencing inhibited cell proliferation in A549 and H1299 cells. (B) Colony formation assay indicated that knockdown of HHLA2 reduced the numbers of colonies 14 days after siHHLA2 transfection. (C) Immunofluorescent staining indicated that Ki67 was downregulated with HHLA2 knockdown. Scale bar: 100 μm. (D, E) HHLA2 silencing induced G0/G1 phase arrest in A549 and H1299 cells. (F) The cell cycle‐related proteins were measured with immunoblotting. *p < 0.05, **p < 0.01, ***p < 0.001