FIGURE 2.

ALOX5 overexpression promotes cell growth and protects gastric cancer cells from chemotherapeutic agents‐induced toxicity. (A and B) Analysis of proliferation of AGS and N87 cells after ALOX5 overexpression performed by BrdU labeling. (C and D) Analysis of colony formation of AGS and N87 cells after ALOX5 overexpression. (E and F) Analysis of migration of AGS and N87 cells after ALOX5 overexpression. Analysis of proliferation (G and H) and apoptosis (I and J) after treatment of 5‐FU and cisplatin in ALOX5‐overexpressing AGS and N87 cells. Results are presented as relative to control. Proliferation and apoptosis assays were assessed after 72 h of drug treatment. 5‐FU at 200 nM and cisplatin at 300 nM were used. *p < 0.05, compared to p‐Vector. #p < 0.05, compared to cisplatin or 5‐FU alone