FIGURE 3.

Addition of 5‐HETE promotes cell growth and protects gastric cancer cells from chemotherapeutic agents‐induced toxicity. (A and B) Analysis of proliferation of AGS and N87 cells after 5‐HETE treatment, as evaluated by BrdU labeling. (C and D) Analysis of colony formation of AGS and N87 cells after 5‐HETE treatment. (E and F) Analysis of migration of AGS and N87 cells after 5‐HETE treatment. Analysis of proliferation (G and H) and apoptosis (I and J) after treatment of 5‐FU and cisplatin in the presence of 5‐HETE in AGS and N87 cells. Results are presented as relative to control. Proliferation and apoptosis assays were assessed after 24 h of drug treatment. 5‐HETE at 500 nM, 5‐FU at 200 nM, and cisplatin at 300 nM were used. *p < 0.05, compared to p‐Vector. #p < 0.05, compared to cisplatin or 5‐FU alone