FIGURE 5.

Alox5 inhibitors suppress gastric cancer and enhance the toxicity of chemotherapeutic agents. (A and B) Proliferation level of AGS and N87 cells after zileuton and AA861 treatment in the presence of 5‐FU and cisplatin, as evaluated by BrdU labeling. (C and D) Proliferation level of AGS and N87 cells after zileuton and AA861 treatment in the presence of 5‐FU and cisplatin, as evaluated by TUNEL assay. Zileuton and AA861 significantly enhance the anti‐proliferative and pro‐apoptotic effects of 5‐FU and cisplatin in gastric cancer cells. 5‐FU at 50 nM and cisplatin at 50 nM were used. Zileuton at 100 μM and 200 μM, AA86 at 30 μM and 60 μM were used in proliferation and apoptosis assays, respectively. Proliferation and apoptosis assays were assessed after 72 h of drug treatment. *p < 0.05, compared to control. #p < 0.05, compared to cisplatin or 5‐FU