FIGURE 7.

ALOX5‐5‐HETE axis activates ERK in gastric cancer cells. (A) Representative western blot photo of p‐Erk, p‐p90RSK, p‐Akt, Mcl‐1, Bim, and Bcl‐2 in N87 cells after ALOX5 overexpression or 5‐HETE addition. (B) Proliferation level of treatment of U0126 and LY2780301 in ALOX5‐overexpressing N87 cells. (C) Proliferation level of treatment of U0126 and LY2780301 in N87 cells in the presence of 5‐HETE. U0126 (MEK inhibitor, 10 μM) but not LY2780301 (Akt inhibitor, 10 μM) significantly reveres the pro‐proliferative effect induced by ALOX5 overexpression and addition of 5‐HETE. Inhibitors were added to the cells at 48h post‐transfection. *p < 0.05, compared to control; ns, not significant