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. 2021 Feb 17;70(7):1536–1548. doi: 10.2337/db20-1108

Figure 1.

Figure 1

HG incubation reduces exosome secretion in BUMPT cells. BUMPT cells were cultured in DMEM containing 30 mmol/L glucose (HG) or 5.5 mmol/L glucose with 24.5 mmol/L d-mannitol (NG) for 8 days as detailed in Research Design and Methods. Cell culture medium was collected at the 8th day for exosome isolation and further analysis. A: NTA of isolated exosomes from the HG condition. B: Immunoblot analysis of exosome marker proteins, including ALIX, CD63, and TSG101. Exosome samples collected from an equal number of cells in the NG and HG groups were analyzed. C: HG incubation reduced exosome secretion. Exosomes were quantified by NTA, and the exosome number was then normalized with the cell numbers in the NG and HG groups (n = 3). **P < 0.01. D: HG treatment did not change the size of exosomes. Peak diameter of exosomes was analyzed by NTA in the NG and HG groups (n = 3).