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. 2021 Feb 17;70(7):1536–1548. doi: 10.2337/db20-1108

Figure 2.

Figure 2

RAB27B is specifically downregulated in HG-treated BUMPT cells. BUMPT cells were cultured in DMEM containing 30 mmol/L glucose (HG) or 5.5 mmol/L glucose with 24.5 mmol/L d-mannitol (NG). Cell lysates were collected for immunoblot analysis. A: Decreased protein level of RAB27B in HG-treated cells. Representative immunoblots of ALIX, TSG101, RAB27A, and RAB27B from cell lysates in the NG and HG groups. Densitometric analyses of ALIX (B), TSG101 (C), RAB27A (D), and RAB27B (E) from cell lysates in the NG and HG groups. β-Actin was used as the loading control. After normalization with β-actin, the protein level in the NG group was arbitrarily set as 1, and the protein level in the HG group was compared with it to calculate the fold change. *P < 0.05 (n = 3). F: Decreased mRNA level of Rab27b in HG-treated cells. The mRNA level of Rab27b was detected by real-time PCR with β-actin as the reference gene. After normalization with β-actin, the mRNA level in the NG group was arbitrarily set as 1, and the mRNA level in the HG group was compared with it to calculate the fold change. *P < 0.05 (n = 3).