Table 2.

Results of immunohistochemistry on MMP-14 in ovarian cancer

Author	Tumour samples	Selection of samples	Years	Country	Scoring system	Number of patients or samples	Result MMP-14	Percentage positive	Conclusion
Sakata et al. [56]	Primary tumours	Selection from archive UHa	n.m	Japan	0–5% = 0, 5–50% = 1, > 50% = 2	114	0 benign, 5 borderline, 59 malignant	76%	More MMP-14 expression in tumours with lymphnode metastasis
Torng et al. [64]	Primary tumours, serous and endometroid	Selection from archive UHa	1993–2000	Taiwan			positive, numbers n.m.b		Expression MMP-14 and MMP-2 correlates
Kamat et al. [57]	Primary tumours	Selection from archive UHa	1990–2000	USA	Overall Score	90	90 epithelial	100, moderate 56%, strong 44%	HRc epithelial MMP-14 2,52 (1.30–4.88)
							87 stromal	97 (62% low, 38% high)	HRc stromal MMP-14 1,30 (1.03–3.39)
									Both MMP-14 epithelial and stromal expression correlates with survival
Lin et al. [59]	Primary tumours	Selection from archive UHa	n.m	USA		77		44% epithelial expression	Strong epithelial MMP-14 expression > 3 risk of death p 0.003
								40% stromal expression
Barbolina et al. [38]	Primary tumours	Selection from archive UHa	n.m	USA		149		78%, 52% high, 94% in clear cell carcinomas	No relation with stage
Adley et al. [26]d	Primary tumours	Selection from archive UHa	1999–2003	USA		143	77	54	32/70 serous, 23/44 endometroid, 5/9 mucinous, 17/18 clear cell
Paulsen et al. [63]	Primary serous borderline tumours and implants	Selection from Cancer Registry (total 632)	1985–1995	Norway		Serous borderline tumours	7 moderate, 33 strong/44, 4 weak, 8 moderate, 42 strong/55		No difference between group with and without implants, all 7 recurrences positive
Brun et al. [60]	Primary serous and mucinous tumours	Selection from archive UHa	2001–2006	France	HSCORE	Serous	115	benign/borderline 99/95, malignant 134
						Mucinous	44
Kato and Motoyama [65]	Primary clear cell and serous carcinomas	Selection from archive UHa	1997–2007	Japan		30 clear cell carcinomas	22	73	No relation with stage or survival
						30 serous carcinomas	1	3
Wang et al. [78]	Primary tumours and metastasis	Selection from archive UHa	2001–2006	China				positive, numbers n.m.c	Colocalization with uPA, numbers not given
Moss et al. [32]	Primary tumours and metastasis	Selection from archive UHa	n.m.b	USA	0–3	15 serous primary	5 weak, 9 moderate, 1 strong	67
						15 serous metastasis	4 weak, 9 moderate, 2 strong	73	Only 2 metastasis had less expression than the primary tumour
						2 endometroid	1 weak	50
						2 endometroid metastasis	2 moderate	100
Brun et al. [60]	Primary tumours	Consecutive series UHa FIGO III-IV	2001–2006	France	HSCORE	43 primary surgery	132 epithelial/12 stromal		No independent prognosticator
						26 interval surgery	97 epithelial/8 stromal
Bruney et al. [49]	Cores of ovarian adenocarcinomas	N.m.b	n.m	USA		50	positive, numbers n.m.b		Inverse relationship with MUC16/CA 125
Nakayama et al. [74]	Primary tumours	Selection from archive UHa	2005–2011	Japan		68	12	18
Trudel et al. [79]	Primary tumours	Selection from archive UHa	1993–2006	Canada	Visual score HSCORE	160/170	105	66	No independent prognosticator
Bruney et al. [48]e	Cores of ovarian adenocarcinomas	N.m.b	n.m	USA		46	positive, numbers n.m.b
Vos et al. [61]	Primary tumours	Consecutive series archives teaching hospital	1997–2003	the Netherlands	Overall Score	94	53 positive epithelial	56	No independent prognosticator
							49 positive stromal	52
Vos et al. [62]	Primary tumours	Consecutive series archives clinical network	2007–2008	the Netherlands	Overall Score	97	52 positive epithelial	54
							5 positive stromal	5
Takahashi et al. [80]	Primary tumours	Selection from archive UHa	2000–2014	Japan	Visual and computer-supported evaluation	107 carcinoma's, 54 borderline, 45 benign	positive, numbers n.m.b		In-situ proximity assay for EphA2 and MMP-14 positive
Vos et al. [58]	Primary tumours and haematogenic and lymphogenic metastasis	Consecutive series archives teaching hospital	2000–2015	the Netherlands	Overall Score	44	37 primary tumours positive/7 missing	37/44	Most metastasis also positive
							34 positive stromal/3 negative stromal/7 missing

^aUH University Hospital

^bn.m. Not mentioned

^cHR Hazard Ratio

^doverlap with study by Barbolina not clear

^eoverlap with earlier Bruney study not clear