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. 2021 Aug 4;20:155. doi: 10.1186/s12934-021-01647-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Biosynthesis of adipic acid pathway intermediates from glucose. Shake-flask cultivation profile of C. glutamicum harboring (a) pZ8-paaJ-tesB and (b) pZ8-paaH-tesB-paaJ. OD₆₀₀ (filled circles), residual sugar (empty circles). N = 3; error bars = standard deviation. c Representative GC/MS ion (m/z = 363) extracted chromatogram of MSTFA-derivatized cultivation broth from C. glutamicum transformed with pZ8-paaH-tesB-paaJ. The peak at retention time 13.3 min corresponds to MSTFA-derivatized 3-hydroxyadipic acid. (d) m/z fragmentation pattern of the peak corresponding to MSTFA-derivatized 3-hydroxyadipic acid from (c)