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. 2021 Jul 14;27(13-14):881–893. doi: 10.1089/ten.tea.2020.0135

FIG. 1.

FIG. 1.

The differentiation of cerebellar spheroids from iPSK3 cells. (A) Schematic illustration of cerebellar differentiation using iPSK3 cells. (B) Representative fluorescent images of day 35 replated cerebellar spheroids for common neuronal markers: (i) GLUT and GABA; (ii) SYN1 (presynaptic) and PSD95 (postsynaptic). Blue: Hoechst. (C) Representative fluorescent images of cerebellar spheroids at day 35 for neural developmental and cerebellar markers: (i) HOXB4 and PAX6; (ii) NEPH3, OLIG2, and MATH1. The NEPH3/Hoe and GLUT/NEPH3/Hoe images were taken using Zeiss LSM 880 confocal microscope, whereas the other images were taken using the Olympus IX70 fluorescent microscope. Scale bar: 100 μm.