FIG. 4.

Electrophysiological properties of the cells from day 80 cerebellar organoids differentiated from iPSK3 line. The day 80 cerebellar organoids were derived with RCP condition. The organoids were replated on Matrigel-coated coverslips and grown for 1 week before the recording. (A) Representative traces of voltage clamp recordings showing fast inward Na⁺ currents followed by long-lasting outward K⁺ currents evoked by depolarizing voltage steps. (B) Representative traces of current clamp recordings showing voltage responses to stepwise current injections. Repetitive action potentials were evoked by depolarizing (+400 pA) current injection, whereas Ih potential was elicited by hyperpolarizing (−320 pA) current injection. (C) Phase contrast image of the patch clamp recoding; scale bar: 50 μm.