TABLE 3.

Stability results for ManNAc, Neu5Ac, and CMP-Neu5Ac in LQC (surrogate blank matrix) and in HQC (blank matrix)

	LQC (75.0 ng/ml)	HQC (8067 ng/ml)
ManNAc	Deviation (%)	Deviation (%)
Room temperature for 18 h	8.7	4.7
Four freeze-thaw cycles	3.7	1.8
Autosampler at 4°C for 67 h	12.4	9.2
Processed sample at 4°C for 71 h	6.5	8.5
Long-term −80°C for 199 days	−1.9	1.4
Neu5Ac	LQC (75.0 ng/ml)	HQC (8975 ng/ml)
	Deviation (%)	Deviation (%)
Room temperature for 18 h	7.1	−5.7
Seven freeze-thaw cycles	−8.7	−13.0
Autosampler at 4°C for 114 h	7.7	3.1
Processed sample at 4°C for 100 h	2.8	−3.1
Long-term −80°C for 198 days	2.1	2.7
CMP-Neu5Ac	LQC (30.0 ng/ml)	HQC (771 ng/ml)
	Deviation (%)	Deviation (%)
Room temperature for 21 h	2.0	−10.9
Five freeze/thaw cycles	−4.3	−3.5
Autosampler at 4°C for 73 h	−2.7	−10.6
Processed sample at 4°C for 73 h	3.7	−9.7
Long-term −80°C for 181 days	0.0	−3.6