Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 3;23:82. doi: 10.1186/s13058-021-01461-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — FGFR4 expression and functional characterization in human breast cancer organoids. a Immunohistochemical staining for FGFR4 across a panel of 9 human breast cancer organoid lines. b Immunohistochemical staining for FGFR4 of the original tumor tissue used to establish the HBC22 organoid line. Panel i) shows staining with the FGFR4 antibody, while ii) is the minus antibody control. c Effect of the FGFR4 inhibitor BLU9931 on organoid growth. Images of DMSO control or BLU9931-treated FGFR4-high HBC22 and FGFR4-low HBC30 organoids at endpoint. d Quantification of (c) comparing the FGFR4-high HBC22 organoid line (red) with the FGFR4-low HBC30 line (blue) normalized to the DMSO control. Statistical significance was determined using an unpaired t test. * indicates p value of < 0.05, ** < 0.01. Error bars: mean ± standard error of three biological replicates, each with three technical replicates