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a SDS-PAGE analysis of purified recombinant GAD. M: protein ladder; 1: intracellular proteins of E. coli BL21 (DE3)/pET28a (+) (vector control); whole-cell intracellular proteins and affinity-purified proteins of E. coli BL21 (DE3)/GADZ1 (lanes 2 and 3), E. coli BL21 (DE3)/GADZ11 (lanes 4 and 5), and E. coli BL21 (DE3)/GADZ20 (lanes 6 and 7). b After incubation of the purified GADZ1, GADZ11, and GADZ20 proteins with l-Glu at pH 5.0, the reaction mixtures were analyzed using HPLC
