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. 2021 May 27;8(15):2100606. doi: 10.1002/advs.202100606

Figure 3.

Figure 3

METTL14 promotes MAVS mRNA decay. A) qPCR analysis of Ifnb1, Mavs, Ddx58, Tbk1, and Irf3 mRNAs in Mettl14 +/+ and Mettl14 +/− peritoneal macrophages, followed by infection with SeV for the indicated times. B) qPCR analysis of Mavs mRNAs (left) and Mavs mRNA degradation (right) in Mettl14 +/+ and Mettl14 +/− peritoneal macrophages infected with SeV for 8 h, followed by treatment with actinomycin‐D for the indicated times. C) qPCR analysis of Mavs mRNAs (left) and Mavs mRNA degradation (right) in Mettl14 +/+ and Mettl14 +/− BMDMs infected with SeV for 8 h, followed by treatment with actinomycin‐D for the indicated times. D) qPCR analysis about the degradation of Mavs mRNA in macrophages reconstituted with empty vector and METTL14 using lentivirus followed by infection with SeV for 8 h and treatment with actinomycin‐D as indicated times. E) qPCR analysis about the degradation of Mavs mRNA in macrophages reconstituted with empty vector, METTL14, and mutant METTL14 (R298P) using lentivirus followed by infection with SeV for 8 h and treatment with actinomycin‐D as indicated times. Data information: Data are presented as mean ± S.D. Two‐tailed unpaired Student's t‐test; *P < 0.05; **P < 0.01 (A–E).