Table 4.

Performance of the commercial cytokine kits

Kit name	Platform	Sample media	Cytokine sources	Number of cytokines	Signal readout	Linear range [pg mL−1]
Bio‐Plex Pro assay	Luminex	Tissue and cell culture supernatants, plasma, and serum	Human	27	Median fluorescence intensity (MFI)	10–104
MILLIPLEX MAP plex kit	Luminex	Human serum, plasma and cell culture supernatants	Human	13	MFI	3.2–104
Invitrogen 25‐plex kit	Luminex	Human serum, plasma and cell culture supernatants	Human	25	MFI	0.5–3.6 × 104
Invitrogen magnetic 30‐plex kit	Luminex	Human serum, plasma and cell culture supernatants	Human	30	MFI	0.5–3.6 × 104
Human enhanced sensitivity 3‐plex kit	Flow cytometry	Tissue culture supernatants, plasma, and serum samples	Human	3	MFI	0.3–2 × 102
Becton Dickinson human Th1/Th2 cytokine kit	Flow cytometry	Tissue culture supernatants, EDTA plasma, and serum samples	Human	14	MFI	Nondetermined −5 × 103
MACSPlex cytokine kit	Flow cytometry	Serum, plasma, and cell culture supernatants	Human	12	MFI	Nondetermined −104
Plex Th cytokine 13‐plex panel	Flow cytometry	Serum and cell culture supernatant samples	Human	13	MFI	Nondetermined −104
U‐PLEX biomarker group 1 (NHP) assays	MSD	Serum, plasma, cell culture supernatant	Nonhuman primates	30	Light signal	Nondetermined −1.8 × 105
Human Th1/Th2 10‐plex Ultra‐Sensitive kit	MSD	Serum, plasma	Human	10	Light signal	Nondetermined −104
Human pro‐inflammatory‐9 ultrasensitive kit	MSD	Serum, plasma	Human	9	Light signal	Nondetermined −105