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. 2021 Jun 3;8(15):2100233. doi: 10.1002/advs.202100233

Figure 5.

Figure 5

HBO reprograms immune TME and helps PD‐1 Ab invigorate CTLs. A) Immunofluorescence staining of MDSCs (CD11b and Gr‐1 colocalization), TAM (M1: CD86, M2: CD206) images and CTL (CD8 and IFN‐γ colocalization) images. The scale bar is 50 µm. Quantification of MDSCs B), ratio of M1/M2 TAMs C), and CTL D) in tumor tissues sections (n = 20). Cytokine levels of E) TGF‐β, F) IL‐10, and G) IL‐2 in serum from H22 orthotopic tumor‐bearing mice analyzed by ELISA (n = 6). Ki67 expression H), CD44 I), IFN‐γ J), granzyme B K), and effector memory T cells L) proportions in H22 orthotopic tumor immediately after various treatments analyzed by flow cytometry. Error bars indicate SEM. Statistical significance was calculated by t‐test. P‐values: *, P < 0.05; **, P < 0.01; ***, P < 0.001.