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. 2021 Jun 3;8(15):2100233. doi: 10.1002/advs.202100233

Figure 6.

Figure 6

HBO synergizes with PD‐1 Ab to inhibit the relapse of the H22 tumor. A) Experimental scheme of HBO helping PD‐1 Ab trigger a long‐term immunological memory effect. B) Tumor growth curves and D) tumor weight of the first tumors (n = 8). C) Tumor formation curves and E) tumor weight of the rechallenged tumors (n = 8). Proportions of effector memory T cells gated on lymphocyte F) and gated on CD8+ T cells G) from spleen analyzed by flow cytometry at day 51 before mice were rechallenged with the secondary tumors (n = 6). IFN‐γ H) and granzyme B I) expression of CD8+ T cells in lymph node from mice isolated 10 days after mice were rechallenged with the secondary tumors (at day 61) (n = 8). Cytokine of J) IL‐2 and K) IFN‐γ in serum from mice isolated 10 days after mice were rechallenged with the secondary tumors (at day 61) (n = 4). Error bars indicate SEM. Statistical significance was calculated by t‐test. P‐values: *, P < 0.05; **, P < 0.01; ***, P < 0.001.