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. 2021 May 30;8(15):2100753. doi: 10.1002/advs.202100753

Figure 2.

Figure 2

The bromodomains of Bdf1 are important for the DNA damage response and DSB repair by HR. A) Scheme showing the conserved motifs in the BRD domains of Bdf1 and its orthologs from indicated species. The two key residues, Y187 and Y354 (marked in red), are critical for maintaining the structure of BRDs. B) DNA damage sensitivity test for indicated strains at indicated drug concentrations. C) Plot showing the relative efficiency of gene integration at the ISU1 locus in indicated strains (n = 4). D) Scheme showing the assay for ectopic recombination repair of the HO‐induced DSB. E) The survival rate of ectopic recombination for the WT and bdf1‐Y2F mutant (n = 4). F,G) Southern blot analysis and quantification of repair kinetics for the WT and the bdf1‐2YF mutant cells (n = 3). H) Plot showing the frequency of YAC loss in indicated cells (n = 3). Data in this figure are presented as mean ± S.D., p‐values are calculated using unpaired two‐tailed Student's t‐test, *p < 0.05, **p < 0.01, ***p < 0.001. n.s. means no significance.