FIGURE 4.

Effect of amiRNA-mediated perturbation of KNU on the expression of meristem regulators. A p35S::KNU-amiRNA transgene was introgressed into pAP1::AP1-GR ap1-1 cal-1 plants. Flower development in this line, as well as in pAP1::AP1-GR ap1-1 cal-1 plants that did not express the KNU-amiRNA, was induced by dexamethasone treatment and floral buds were collected 5, 6, and 7 days after the treatment. Transcript levels of different meristem regulators (as indicated) were determined by RT-qPCR in four (5 and 6 days) or three (7 days) biologically independent sets of samples. Fold change expression values in the amiRNA-expressing lines relative to the lines with normal KNU activity are shown. Bars indicate SEM.