Table 2:
TTN values for geranylation reaction with FamD2.a
Compound | TTN Value |
---|---|
1 | 1495 |
6 | 1615 |
7 | N/Ab |
8 | 1481 |
9 | 1902 |
10 | 947 |
11 | 1547 |
12 | 1625 |
13 | 803 |
14 | 755 |
15 | 1174 |
16 | 710 |
17 | 1281 |
18 | 1366 |
19 | 1454 |
Assay conditions: 1 μM FamD2, 2 mM substrate, 1.5 mM GPP, 5 mM MgCl2, 50 mM glycine (pH 10.0), 100 μL, 37°C, 200 rpm, 1 hr.
Compound 7 showed no conversion in assay