Figure 1.

α2-Chimaerin coprecipitates with cytoskeletal regulatory proteins and mutations alter the pattern of these interactions. A, Venn diagram of the proteins identified from immunoprecipitation of the fusion proteins YFP-α2-CHN-WT (WT), YFP-α2-CHN-L20F (L20F), or YFP-α2-CHN-G228S (G228S) and subsequent mass spectrometry. Proteins shown were identified with high confidence in at least two of three samples of the triplicate. The size of each circle corresponds with the total number of proteins identified in each sample. B–D, String (https://string-db.org/) interactome analysis of the proteins included in A, showing the interactions described between the proteins present in the sample and based on data mining; the thickness of the connecting lines represents the strength of the supporting evidence for the interaction. Color coding as in E. E, Pathway ID and functional enrichment analysis of each protein sample, showing the false discovery rate for and the pathway ID of the three biological processes shown in the data.