Figure 2.

α2-Chimaerin interacts with STMN1/2. Input and coimmunoprecipitation of STMN1 and STMN2 in HEK293T cells cotransfected with YFP, or the fusion proteins YFP-α2-CHN-WT (WT), YFP-α2-CHN-L20F (L20F), or YFP-α2-CHN-G228S (G228S) with RFP-tagged STMN1 or STMN2. Immunoprecipitation was performed using anti-GFP antibodies. Membranes were blotted using anti-DsRed, anti-GFP, and anti-GAPDH antibodies. STMN1 coprecipitates with the G228S isoform of α2-CHN, while STMN2 coprecipitates with both isoforms. RFP-tagged STMN1 is ∼45 kDa (asterisk), and STMN2 is 48 kDa (dash). The sizes are indicated on the blot.