Figure 3.

α2-Chimaerin colocalizes with CRMP2 and stathmin 1/2 in rat cortical neurons. Immunocytochemistry and imaging of YFP-α2-CHN-transfected cells. A–C, Detection of CRMP2 in transfected YFP-positive cells. DAPI signal from cell body is shown in yellow, YFP is shown in cyan, and CRMP2 is shown in magenta. A, A′, Double immunocytochemistry detection of YFP-α2-CHN and CRMP2. B, B′, Negative control of PLA in the absence of CRMP2 primary antibody. C, C′, Proximity ligation assay of CRMP2 in a YFP-positive cell. D, D′, Similar panels for STMN1 immunocytochemistry with STMN1 in magenta. E, F′, Negative control (E, E′) and PLA (F, F′). G, G′, Similar panels for STMN2 immunocytochemistry with STMN2 magenta. H–I′. Negative control (H, H′) and PLA (I, I′). White arrowheads in C′, F′, and I′ denote colocalization of the proteins in cell bodies, and open arrowheads in the same panels denote colocalization in axons. Scale bars, 50 µm.