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. 2021 Aug 4;41(31):6652–6672. doi: 10.1523/JNEUROSCI.0983-19.2021

Figure 4.

Figure 4.

Expression of dpysl2 and stmn1/2 in the ocular motor system of the larval zebrafish. A, Schematic representation of the dorsal view of CN III (oculomotor) and CN IV (trochlear) nuclei at 48 hpf. Midline and midbrain–hindbrain boundary are shown, with anterior (A) and lateral (L) axes. B, Fluorescent signal corresponding to the negative control probe following RNAscope in situ hybridization in Tg(isl1BAC:Gal4;UAS:GFP) larvae. Anti-GFP immunohistochemistry (cyan) was used to counterstain and highlight the cranial motor neurons of the oculomotor nerve (CN III) and trochlear nerve (CN IV), shown by dotted lines. C–G, Fluorescent signal following RNAscope in situ hybridization is shown in magenta using probes against dpysl2 (C), stmn1a (D), stmn1b (E), stmn2a (F), and stmn2b (G). Scale bars, 50 µm.