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. 2021 Aug 4;41(31):6652–6672. doi: 10.1523/JNEUROSCI.0983-19.2021

Figure 6.

Figure 6.

Knockdown of dpysl2 and stmn1/2 leads to defects in OMS development. Neurite tracer-extracted image following whole-mount staining of the OMS of 3 dpf wild-type zebrafish larvae using anti-tubulin (cyan) and anti-myosin heavy chain (magenta). Abbreviations are as in Figure 5. Ai–Diii, Representative images of the OMS of larvae following injection of standard (A), dpysl2 (B), stmn1a + stmn1b (C), and stmn2a + stmn2b morpholinos (D). Yellow arrows indicate ectopic branching, and white arrows indicate defasciculation. E, Quantification of the average defasciculation and ectopic branching of the OMS shown as a percentage. F, Quantification of the defasciculation events (percentage) analyzed in the main bundle of the OMN, each of the four branches of the OMN, and trochlear and abducens nerves. G, Quantification of the ectopic branching events (percentage) analyzed in each branch. Standard MO, n = 17; dpysl2 MO, n = 20; stmn1a + stmn1b, n = 19; stmn2a + stmn2b, n = 15. Mann–Whitney test for comparison of phenotypes; **p < 0,005, ***p < 0.0005. Scale bars: Ai, Bi, Ci, Di, 100 µm; Aii, Aiii, Bii, Biii, Cii, Ciii, Dii, Diii, 20 µm.