Figure 7.

Extraocular muscles and cranial nuclei of the OMS develop normally in morphants. A, Schematic representation of the position and organization of the six extraocular muscles (magenta). Abbreviations are as in Figure 5. B–F, Representative images following whole-mount immunostaining of the EOMs, using an anti-myosin heavy chain antibody (magenta) at 72 hpf: wild-type, noninjected larvae (B), standard (C), chn1 (D), dpysl2 (E), and stmn1a + stmn1b + stmn2a + stmn2b (F) morpholino-injected larvae. G, Schematic representation of the position and organization of the nuclei of the oculomotor (CN III) and trochlear (CN IV) cranial nerves of the OMS as visualized in Tg(isl1BAC:Gal4;UAS:GFP) larvae. H–L, Representative dorsal view images of the GFP signal at 3 dpf shown in grayscale; dotted lines highlight CN III and CN IV: wild-type, noninjected larvae (H); standard (I), chn1 (J), dpysl2 (K), and stmn1a + stmn1b + stmn2a + stmn2b (L) morpholino-injected larvae. Although there is some variability in the level of GFP expression, the overall layout is unaffected. Scale bars, 100 µm.