Figure 8.

Overexpression of CHN1, CRMP2, and STMN1 rescues chn1 knock-down phenotypes. Representative neurite tracer-extracted images following whole-mount staining of the OMS of 3 dpf wild-type zebrafish larvae using anti-tubulin (cyan) and anti-myosin heavy chain (magenta). A–C, E, G, Representative images of larvae following the injection of standard MO (A), chn1 MO (B), chn1 MO with CHN1 RNA (C), chn1 MO with dpysl2 RNA (E), and chn1 MO with YFP-STMN1 RNA (G). A–C, E, G, Insets in i show higher magnification of observed defasciculation (white arrows) of specific nerve branches in ii and iii. D, Quantification of the average defasciculation and ectopic branching across the OMS shown as a percentage, comparing larvae injected with standard MO with GFP RNA, chn1 MO with GFP mRNA, and chn1 MO with CHN1 RNA. Standard MO, n = 36; chn1 MO, n = 19; and chn1 MO with CHN1 RNA, n = 40. F, Quantification of the average defasciculation and ectopic branching across the OMS shown as a percentage, comparing standard MO, chn1 MO, and chn1 MO with dpysl2 RNA-injected larvae. Standard MO, n = 17; chn1 MO, n = 23; and chn1 MO with dpysl2 RNA, n = 13. H, Quantification of the average defasciculation and ectopic branching across the OMS shown as a percentage, comparing standard MO-injected embryos with embryos injected with GFP RNA, chn1 MO with GFP RNA, and chn1 MO with YFP-STMN1 RNA. Standard MO + GFP RNA, n = 35; chn1 MO + GFP RNA, n = 15; chn1 MO + STMN1 RNA, n = 17. Mann–Whitney test for comparison of phenotypes: *p < 0.05, **p < 0.005, ***p < 0.0005. Scale bars: Ai, Ci, Ei, Gi, 100 µm; Aii, Aiii, Cii, Ciii, Eii, Eiii, Gii, Giii, 20 µm.