Figure 9.

chn1 MO-injected zebrafish larvae show reduced optokinetic reflex. A, Schematic representation of the OKR setup. B, Diagram of zebrafish larval eye movements showing abduction and adduction movements for left and right eye. C, Effect of the chn1 MO injection on the abduction and adduction range of movements separated into dominant and weak eyes. In all cases, injected fish showed higher variability and a reduction in adduction. D, Similar results observed in the case of saccadic velocity. E, F, Gain curves for abduction (E) and adduction (F) movements. chn1 MO-injected fish show lower gain levels, especially when comparing weak eyes. Range and saccadic velocity compared with t test; gain was compared using two-factor ANOVA with replication: **p < 0.01. Noninjected, n = 13; chn1 MO, n = 13.