Table 1.
Quantification of observed neuroanatomical phenotypes in the ocular motor system following morpholino knockdown
| Oculomotor nerve |
Trochlear nerve |
Abducens nerve |
Total |
N | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Main bundle |
Superior rectus |
Inferior rectus |
Medial rectus |
Inferior oblique |
|||||||||||||
| % | p value | % | p value | % | p value | % | p value | % | p value | % | p value | % | p value | % | p value | ||
| Defasciculation | |||||||||||||||||
| Standard MO | 41% | 47% | 18% | 12% | 35% | 6% | 18% | 25% | 17 | ||||||||
| Noninjected | 38% | 1.0000 | 29% | 0.3281 | 25% | 0.7113 | 13% | 1.0000 | 63% | 0.1180 | 16% | 0.6052 | 10% | 0.6396 | 28% | 1.0000 | 25 |
| chn1 MO | 58% | 0.3580 | 42% | 1.0000 | 52% | 0.0504 | 44% | 0.0414 | 77% | 0.0105 | 23% | 0.2056 | 32% | 0.4644 | 48% | 0.0034 | 23 |
| dpysl2 MO | 84% | 0.0070 | 70% | 0.1991 | 46% | 0.0958 | 64% | 0.0025 | 72% | 0.0274 | 7% | 1.000 | 20% | 1.000 | 55% | 0.0009 | 20 |
| stmn1a MO | 91% | 0.0161 | 82% | 0.1150 | 45% | 0.1998 | 64% | 0.0104 | 89% | 0.0145 | 36% | 0.0618 | 18% | 1.000 | 60% | <0.0001 | 11 |
| stmn1a + 1b MO | 74% | 0.0895 | 79% | 0.0819 | 53% | 0.0411 | 63% | 0.0022 | 53% | 0.3351 | 56% | 0.0027 | 74% | 0.0011 | 64% | <0.0001 | 19 |
| stmn2a MO | 67% | 0.2635 | 100% | 0.0033 | 83% | 0.0007 | 67% | 0.0045 | 90% | 0.0140 | 33% | 0.1296 | 75% | 0.0032 | 73% | <0.0001 | 12 |
| stmn2a + 2b MO | 100% | 0.0008 | 89% | 0.0116 | 61% | 0.0189 | 53% | 0.0275 | 89% | 0.0054 | 53% | 0.0024 | 60% | 0.0324 | 73% | <0.0001 | 15 |
| stmn1a + 1b + 2a + 2b MO | 88% | 0.0075 | 79% | 0.1014 | 57% | 0.0411 | 43% | 0.0365 | 78% | 0.0166 | 50% | 0.0072 | 54% | 0.0176 | 64% | <0.0001 | 24 |
| Ectopic branching | |||||||||||||||||
| Standard MO | 12% | 0% | 0% | 12% | 0% | 0% | 0% | 3% | 17 | ||||||||
| Noninjected | 7% | 1.0000 | 0% | 1.0000 | 17% | 0.1284 | 14% | 1.0000 | 4% | 1.0000 | 0% | 0% | 6% | 0.2660 | 25 | ||
| chn1 MO | 65% | 0.0004 | 38% | 0.0072 | 28% | 0.0300 | 24% | 0.4388 | 4% | 1.0000 | 0% | 0% | 24% | 0.0002 | 23 | ||
| dpysl2 MO | 24% | 0.4388 | 30% | 0.0295 | 29% | 0.0295 | 27% | 0.4260 | 88% | 0.0002 | 0% | 0% | 31% | <0.0001 | 20 | ||
| stmn1a MO | 36% | 0.1741 | 36% | 0.0161 | 0% | 1.0000 | 0% | 0.5053 | 22% | 0.1108 | 0% | 0% | 13% | 0.0047 | 11 | ||
| stmn1a + 1b MO | 58% | 0.0058 | 5% | 1.0000 | 11% | 0.4873 | 5% | 0.5929 | 16% | 0.2310 | 0% | 0% | 14% | 0.0008 | 19 | ||
| stmn2a MO | 17% | 1.0000 | 67% | 0.0001 | 8% | 0.4138 | 17% | 1.0000 | 20% | 0.1282 | 0% | 0% | 18% | <0.0001 | 12 | ||
| stmn2a + 2b MO | 83% | <0.0001 | 17% | 0.2286 | 17% | 0.2310 | 0% | 0.2286 | 22% | 0.1062 | 0% | 0% | 21% | <0.0001 | 15 | ||
| stmn1a + 1b + 2a + 2b MO | 50% | 0.0140 | 38% | 0.0094 | 13% | 0.4873 | 9% | 0.5843 | 30% | 0.0473 | 0% | 0% | 20% | <0.0001 | 24 | ||
Quantification is given for the total ocular motor system (averaged across scores for each component) as well as the main bundle and all four branches of the oculomotor nerve, the trochlear nerve, and the abducens nerve individually. Each of the chn1, dpysl2, stmn1, and stmn2 morpholino injections was compared with the standard MO (shown in italic) using the Mann–Whitney test. Exact p values for each comparison are given. P values that are significant are denoted in italics, and higher levels of significance are shown in bold.