Table 2.
Quantification of observed neuroanatomical phenotypes in the ocular motor system following morpholino knockdown and mRNA overexpression
| Defasciculation | Ectopic branches | ||||||
|---|---|---|---|---|---|---|---|
| chn1 rescue | chn1 rescue | ||||||
| Standard MO + GFP RNA | 20% | N = 36 | Standard MO + GFP RNA | 3% | N = 36 | ||
| chn1 MO + GFP RNA | 33% | N = 19 | chn1 MO + GFP RNA | 20% | N = 19 | ||
| chn1 MO + chn1 RNA | 22% | N = 40 | chn1 MO + CHN1 RNA | 3% | N = 40 | ||
| p value | p value | ||||||
| Standard MO + GFP RNA | vs | chn1 MO + GFP RNA | 0.0004 | Standard MO + GFP RNA | vs | chn1 MO + GFP RNA | <0.0001 |
| Standard MO + GFP RNA | vs | chn1 MO + CHN1 RNA | 0.3321 | Standard MO + GFP RNA | vs | chn1 MO + CHN1 RNA | 1.0000 |
| chn1 MO + GFP RNA | vs | chn1 MO + CHN1 RNA | 0.0035 | chn1 MO + GFP RNA | vs | chn1 MO + CHN1 RNA | <0.0001 |
| Standard MO | 25% | N = 17 | Standard MO | 3% | N = 17 | ||
| chn1 MO | 48% | N = 23 | chn1 MO | 24% | N = 23 | ||
| dpysl2 rescue | dpysl2 rescue | ||||||
| chn1 MO + dpysl2 RNA | 27% | N = 13 | chn1 MO + dpysl2 RNA | 9% | N = 13 | ||
| p value | p value | ||||||
| Standard MO | vs | chn1 MO | 0.0034 | Standard MO | vs | chn1 MO | 0.0002 |
| Standard MO | vs | chn1 MO + dpysl2 RNA | 0.5760 | Standard MO | vs | chn1 MO + dpysl2 RNA | 0.2170 |
| chn1 MO | vs | chn1 MO + dpysl2 RNA | 0.0101 | chn1 MO | vs | chn1 MO + dpysl2 RNA | 0.0331 |
| stmn1 rescue | stmn1 rescue | ||||||
| Standard MO + GFP RNA | 29% | N = 35 | Standard MO + GFP RNA | 4% | N = 35 | ||
| chn1 MO + GFP RNA | 49% | N = 15 | chn1 MO + GFP RNA | 17% | N = 15 | ||
| chn1 MO + STMN1 RNA | 35% | N = 17 | chn1 MO + STMN1 RNA | 8% | N = 17 | ||
| p value | p value | ||||||
| Standard MO + GFP RNA | vs | chn1 MO + GFP RNA | 0.0003 | Standard MO + GFP RNA | vs | chn1 MO + GFP RNA | 0.0019 |
| Standard MO + GFP RNA | vs | chn1 MO + STMN1 RNA | 0.0982 | Standard MO + GFP RNA | vs | chn1 MO + STMN1 RNA | 0.4270 |
| chn1 MO + GFP RNA | vs | chn1 MO + STMN1 RNA | 0.0367 | chn1 MO + GFP RNA | vs | chn1 MO + STMN1 RNA | 0.0358 |
Quantification given for the whole ocular motor system, averaged across scores for each component. Coinjection of standard MO and GFP mRNA, chn1 MO and GFP mRNA, and chn1 MO and CHN1 mRNA are each compared to assess the success of the rescue. chn1 MO coinjected with dpysl2 mRNA was compared with standard MO or chn1 MO on their own. Coinjection of chn1 MO and STMN1 mRNA was compared with coinjections of standard MO and GFP mRNA, and chn1 MO and GFP mRNA. Comparisons were performed using Mann–Whitney test; p values are given. P values that are significant are denoted in italics, and higher levels of significance are shown in bold.