Fig. 4.
A schematic illustration of the flow-cell manufacturing process. (a) Double sided tape is placed on a pre-cleaned glass microscope slide containing 1 inlet and 1 outlet hole for sample injection. A pre-cleaned glass microscope coverslip is added directly on top of the double-sided tape to form a chamber. (b) After coating the interior of the chamber with poly-l-lysine and the injection of pre-formed Aβ555 complexes, the fluidic chamber is placed coverslip down onto an oil immersion objective lens housed in an inverted microscope to enable monitoring of surface immobilized oligomers.