. 2021 Aug 4;16(8):e0255651. doi: 10.1371/journal.pone.0255651

Table 2. qPCR primer information and predicted product size.

Target bacterial group	Primer sequences (5’-3’)	Predicted PCR product size (bp)	Annealing temp (°C)	Reference
Total bacteria	UniF: `GTGCTGCATGGTCGTCGTCA`	148	60	[22]
Total bacteria	UniR: `ACGTCGTCCACACCTTCCTC`	148	60	[22]
Romboutsia spp.	F: `TGACATCCTTTTGACCTCTC`	282	54	[23]
Romboutsia spp.	R: `GCCTCACGACTTGGCTG`	282	54	[23]
Lactobacillus spp.	F: `AGCAGTAGGGAATCTTCCA`	341	58	[24]
Lactobacillus spp.	R: `CACCGCTACACATGGAG`	341	58	[24]
Turicibacter spp.	F: `CAGACGGGGACAACGATTGGA`	141	63	[25]
Turicibacter spp.	R: `TACGCATCGTCGCCTTGGTA`	141	63	[25]
Akkermansia spp.	F: `CAGCACGTGAAGGTGGGGAC`	329	60	[26]
Akkermansia spp.	R: `CCTTGCGGTTGGCTTCAGAT`	329	60	[26]
Escherichia coli	F: `CATGCCGCGTGTATGAAGAA`	96	57.5	[27]
Escherichia coli	R: `CGGGTAACGTCAATGAGCAAA`	96	57.5	[27]
Bacteroides spp.	F: `CTGAACCAGCCAAGTAGCG`	230	53	[28]
Bacteroides spp.	R: `CCGCAAACTTTCACAACTGACTTA`	230	53	[28]