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Neighbor-joining tree based on the 373-nucleotide sequence of the ITS-1/5.8S/ITS-2 marker, confirming the identity of amphistomes from Mpumalanga province (in bold) as Calicophoron microbothrium and their relationship with Calicophoron species obtained from the NCBI GenBank database. The support value indicated on the nodes follows the order neighbor-joining bootstrap value/maximum likelihood bootstrap value/Bayesian inference posterior probability.
