Table 1.

Primary antibodies used in immunohistochemistry of anterior pituitary hormones.

Antigen	Animal/clonality	Cat. no./clone	Dilution	Pretreatment	Source
GH	Mouse/mAb	sc-374266	1 : 100	NN	Santa Cruz Biotechnology
ACTH	Mouse/mAb	AM32828PU-N	1 : 200	NN	Acris Antibodies
LH	Mouse/mAb	MS-9078-P1	1 : 1000	MW	Lab Vision
FSH	Mouse/mAb	MS-1449-P1	1 : 500	MW	Lab Vision
TSH	Mouse/mAb	MS-1453-P1	1 : 200	MW	Lab Vision
PRL	Mouse/mAb	MS-9083-P1	1 : 200	MW	Lab Vision

GH, growth hormone; ACTH, adrenocorticotropic hormone; LH, luteinizing hormone; FSH, follicle-stimulating hormone; TSH, thyroid-stimulating hormone; PRL, prolactin; mAb, monoclonal antibody; MW, microwaving in citrate buffer, pH 6.0 (15 min, 95°C, 400 W); NN, not necessary.