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. 2021 Jul 21;596(7870):126–132. doi: 10.1038/s41586-021-03752-4

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — TIL from patient MD01-004 were cultured with MD01-004-MANA-12 or influenza A peptide and titrating concentrations of recombinant human IL-7, followed by coupled scRNA-seq–TCR-seq. A total of 814 influenza-specific (410 co-cultured with influenza peptide, 404 co-cultured with MANA peptide) and 581 MANA-specific TIL (366 co-cultured with influenza peptide, 215 co-cultured with MANA peptide) were detected in the single-cell data from a single experiment and were analysed. a, Composite expression of an IL-7 gene set by influenza-specific and MANA-specific TIL (as determined by their TCR Vβ CDR3) stimulated with cognate or non-cognate antigen is shown. b, Dose–response curve showing the fold change of averaged expression of IL-7-induced genes (Supplementary Data 2.3) that significantly changed from baseline (no IL-7 vs 0.1 ng/ml) in influenza-specific (red) or MANA-specific (blue) T cells. Comparisons were performed using two-sided Wilcoxon rank sum test and adjusted for multiple comparisons using BH method.