Table 2:

Quantitative Fluorescent Image Analysis

Parameter	Trastuzumab	Trastuzumab:1HE	p-value
Positive Trastuzumab Area (SD)	26.52% (±8.11%)	43.32% (±11.42%)	0.0024
Mean Trastuzumab Fluorescence (SD)	13 MFI (±3)	15 MFI (±4)	0.25
Mean Trastuzumab Fluorescence for pixels above 10 (SD)	34 MFI (±5)	27 MFI (±3)	0.0034
Penetration Distance Limit (SD)	68.60 μm (±15.65 μm)	64.54 μm (±5.04 μm)	0.47
Grayscale Penetration Distance (SD)	39.38 μm (±5.89 μm)	51.52 μm (±5.15 μm)	0.0003
Percent of Grayscale to Limit (SD)	58.39% (±5.70%)	79.97% (±7.00%)	<0.0001
Threshold Penetration Distance (SD)	41.30 μm (±6.70 μm)	58.24 μm (±5.40 μm)	<0.0001
Percent of Threshold to Limit (SD)	61.15% (±6.26%)	90.34% (±6.02%)	<0.0001