Fig. 1 |. Overview of prenatal cerebellar development and the data generated in this study.

a, Midsagittal sections of the human fetal cerebellum stained with hematoxylin and eosin (H&E) or markers for Purkinje cells (Calbindin) or rhombic lip (RL) and external granule cell layer (EGL; PAX6 and KI67). A minimum of 2 samples per age were stained with adjacent sections used for histology and immunocytochemistry. The ventricular zone (VZ), RL, EGL, and Purkinje cell layer (PCL) are shown. Arrowheads mark the anterior (yellow) and posterior (white) EGL across the dorsal surface of the cerebellar anlage. At 9 PCW, the cerebellar anlage is dominated by Purkinje cells, with a thin nascent EGL extending from the RL. By 19 PCW, Purkinje cells have migrated radially to establish a multicellular layer (PCL) beneath the EGL. Scale bars: 100 um (grey), 500 um (red), 1 mm (blue). 10 PCW H&E section was used previously in Fig. 1 of Haldipur et al., 2019. b, Schematic illustrating LCM (left) and SPLiT-seq (right) experimental workflows. c, The time span of fetal cerebellar development represented by line drawings of midsagittal sections of the cerebellum (to-scale) showing a dramatic change in size and foliation from 9 to 19 PCW. Below is the distribution of cerebella in this study. Biological and technical replicate samples are not shown (RNA-seq sample numbers: n = 13 for bulk; 9 for RL; 17 for EGL; 18 for PCL; snRNA-seq sample numbers: n = 6 for Exp 1; 11 for Exp 2; 9 for Exp 3).