Fig. 3.

MT1JP confers poor Lenvatinib response on HCC cells through inhibiting apoptosis. a. LR-7721, LR-Huh7 and parental HCC cells were transfected with either control vector (siCon) or two siRNAs targeting MT1JP (siMT1JP-1 and siMT1JP-2) and incubated for 48 h in the absence or presence of Lenvatinib (0.5 μM) (Con and Len). The transfection efficiencies were confirmed by qRT-PCR. b. Viabilities (%) of the above cells (a) treated with or without Lenvatinib (Len and Con) were calculated (n = 3). c. Representative images taken from the above cells (a) incubated with or without Lenvatinib and stained by TUNEL (red) and DAPI (blue) (Scale bars: 10 μm). d. Quantification of TUNEL positive cells in (c). e. Lysates from the above cells (a) incubated with or without Lenvatinib were subjected to Western blot analysis. Band densities were normalized to GADPH. Compared using Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001