Fig. 4.

MT1JP acts as a ceRNA for miR-24-3p to regulate the apoptotic signaling pathway. a. Venn diagram showing overlapping miRNAs that are predicted to bind to MT1JP. b. Expression analysis of miR-24-3p measured in Lenvatinib resistant and parental HCC cells by qRT-PCR. c. Binding sequences of MT1JP and miR-24-3p based on bioinformatics analysis and binding sites of WT (WT-MT1JP) and mutant (Mut-MT1JP) regions. d. Dual-luciferase reporter assays to examine the potential interaction of MT1JP and miR-24-3 (n = 4). e-g. SMMC-7721 and Huh7 cells were transfected with miR-NC (NC) or miR-24-3p inhibitor (inhibitor), while LR-7721 and LR-Huh7 cells were transfected with miR-NC (NC) or miR-24-3p mimics (mimics). Apoptosis through TUNEL (e-f) and Western blotting (g) assays was detected in these transfected cell lines. (Scale bars: 10 μm). Data represent three independent experiments. Compared using Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001