Fig. 5.

BCL2L2 suppression, mediated by miR-24-3p, is involved in MT1JP-mediated Lenvatinib tolerance. a. Venn diagram showing overlapping genes that are predicted to bind to miR-24-3p. b. GO enrichment graph of the common 171 genes predicted in (a). P values (white) and different gene numbers (black) are listed in the graph. c. HCC cell lines were transfected with either MT1JP vector or miR-24-3p mimics. Expression of BCL2L2 was detected by Western blotting. d-e. SMMC-7721 and Huh7 cells were transfected with control vector (vector) or BCL2L2, while LR-7721 and LR-Huh7 cells were transfected with control siRNA (siNC) or BCL2L2 siRNA (siBCL2L2). The viability (%) of these transfected cells incubated with Lenvatinib (5 μM) was calculated. f-h. Apoptosis through TUNEL (f-g) and Western blotting (h) assays was detected in the above cells (e). Data represent three independent experiments. (Scale bars: 10 μm). Compared using Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001